SPECIES
EXPERIMENT NAME
SHORT DESCRIPTION
LONG DESCRIPTION
Methanococcus jannaschii
(DSM 2661)
MJ_LOHLow hydrogenCells were grown in a fermenter. Control cells were collected at exponential growth phase. The partial pressure of hydrogen was changed from 10kPa to 1kPa and cells were collected 2 hours later. The cells were still in exponential growth phase.
Methanococcus jannaschii
(DSM 2661)
MJ_CLDSKCold shockCells were grown in a fermenter. Control cells were collected at exponential growth phase. The temperature was lowered from 83 °C to 68 °C, and cells were collected 2 hours later. The cells were still in exponential growth phase.
Methanococcus jannaschii
(DSM 2661)
MJ_HTSKHeat shockCells were grown in a fermenter. Control cells were collected at exponential growth phase. The temperature was increased from 83 °C to 93 °C, and cells were collected 1 hour later. The cells were still in exponential growth phase.
Methanococcus jannaschii
(DSM 2661)
MJ_901VMW profiling
Methanococcus jannaschii
(DSM 2661)
MJ_AMMON3Ammonia limitation (3hrs)Cells were inoculated from a bottle at 2.2 mM NH4 (10-fold lower than normal) to a fermenter at 0.44 mM (50-fold lower) and cells were allowed to grow for a short time. Then, pretreatment cells were collected (exponential growth phase), and NH4Cl concentration was increased to 22 mM (normal). Cells were collected 3 hours later. The cells were still in exponential growth phase.
Methanococcus jannaschii
(DSM 2661)
MJ_AMMON5Ammonia limitation (5hrs)Cells were inoculated from a bottle at 2.2 mM NH4 (10-fold lower than normal) to a fermenter at 0.44 mM (50-fold lower) and cells were allowed to grow for a short time. Then, pretreatment cells were collected (exponential growth phase), and NH4Cl concentration was increased to 22 mM (normal). Cells were collected 5 hours later. The cells were in early stationary phase at this time point.
Pyrococcus furiosusN994_40The effect of sulfurTwo groups of cells were grown in fermenters. Both groups were grown on 0.5% maltose, but one group also was grown with 0.1% (w/v) elemental sulfur. Cells were collected in exponential growth phase. The samples were TCA-precipitated prior to separation.
Pyrococcus furiosusPF_MALTSULThe effect of sulfurTwo groups of cells were grown in fermenters. Both groups were grown on 0.5% maltose and 0.05% yeast extract, but one group also was grown with 0.1% (w/v) elemental sulfur. Cells were collected in exponential growth phase.
Pyrococcus furiosusPF_CARBPEPTMaltose vs. CaseinTwo groups of cells were grown in fermenters. One group was grown on 0.5% maltose (carbohydrate), 0.05% yeast extract, and 0.1% sulfur. The other group was grown on 0.5% casein hydrolysate (peptide carbon source), 0.05% yeast extract, and 0.1% sulfur.
Pyrococcus furiosusPFMEMIPG1
Pyrococcus furiosusPFWCEIPG1

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